江汉大学学报（自然科学版）

目的建立苯妥英钠的血药浓度测定方法,帮助临床合理用药。方法血清样品采用固相萃取,用高效液相色谱法测定。色谱柱为Venusil C18,流动相为甲醇∶水(50∶50),流速0.8 m L/min,检测波长230 nm。结果本方法中内源性物质不干扰测定,苯妥英钠在2.84~181.80μg/m L范围内线性关系良好(R=0.999 4),检测限为0.6μg/m L;低、中、高浓度平均回收率为92.7%~96.2%,日内和日间精密度RSD均小于10%。结论本方法专属、准确、灵敏,适用于苯妥英钠的血药浓度测定和相关药动学研究。

Objective To establish a method for the determination of phenytoin sodium in serum to assist rational administration in clinic. Methods Serum samples were extracted with solid phase extraction(SPE),and phenytoin sodium was detected with high performance liquid chromatography(HPLC).The separation was performed on a Venusil C18 column with a mobile phase of methanol∶water(50∶50)at a flow rate of 0. 8 m L/min. The eluent was monitored at 230 nm. Results The method showed a goodspecificity between analyte and matrix. A good linearity of phenytoin sodium was observed over theconcentration range of 2. 84-181. 80 μg/m L with correlation coefficient of 0. 999 4. The limits ofdetection(LOD)was 0. 6 μg/m L. The mean recovery of phenytoin sodium in quality control(QC)samples at concentrations of 2. 84,22. 70,181. 80μg/m L were between 92. 7%-96. 2%,intra-andinter-day precision RSD were both less than 10%( n = 5). Conclusion The proposed method isspecific,accurate and sensitive. The results indicate that the method is satisfactorily applied to detectthe serum phenytoin sodium concentrations,and to perform related pharmacokinetics studies.