江汉大学学报（自然科学版）

研究冬瓜多糖的超声波辅助提取工艺及体外抗氧化活性。在单因素试验的基础上,探究料液比、提取时间、提取功率对冬瓜多糖提取率的影响,然后以正交试验确定其适宜提取工艺。通过对超声波辅助提取所提得冬瓜多糖的DPPH自由基、羟基自由基、超氧阴离子自由基的清除能力进行测定,来表征冬瓜多糖的体外抗氧化活性。结果表明:超声波提取时间及料液比对冬瓜多糖提取率的影响显著(P<0.05),超声波辅助提取冬瓜多糖适宜工艺条件为,料液比1∶40,超声波提取时间40 min,提取功率380 W,此条件下冬瓜粗多糖得率为13.15%,相比于热水浸提法,得率提高50%以上,提取时间缩短1/2。当冬瓜多糖浓度为5 mg/mL时,其对DPPH自由基、羟基自由基、超氧阴离子自由基的清除率分别为77.91%、99.64%、74.48%。超声波辅助提取能显著提高冬瓜多糖的提取效率,且冬瓜多糖具有较好的抗氧化活性。

The ultrasonic-assisted extracted technique for wax gourd polysaccharide and the antioxida-tive activity of polysaccharides were studied in this paper.On the basis of single factor experiment,thiswork studied the effects of solid to liquid ratio,extraction time,and extraction power on the extractionyield of wax gourd polysaccharide.Then the optimal extraction conditions were obtained by the orthogo-nal experiment.Finally,the in vitro antioxidant ability of wax gourd polysaccharide was characterized bystudying its reactivity to DPPH radical,hydroxyl radical,superoxide anion radical.The influences ofultrasonic extraction time and solid to liquid ratio on the extraction rate of wax gourd polysaccharidewere significantly(P < 0.05).The suitable ultrasonic-assisted extraction conditions for wax gourd poly-saccharide were solid to liquid ratio 1∶40,ultrasonic extraction time 40 min,extraction power 380 W,under the conditions,the yield of crude wax gourd polysaccharide was 13.15%,compared with the hotwater extraction method,the yield increased by more than 50%,the extraction time shorten by 1/2.When the concentration of wax gourd polysaccharides was 5 mg/mL,the clearance rates of DPPH radi-cal,hydroxyl radical and superoxide anion radical were 77.91%,99.64% and 74.48%,respectively.The ultrasonic-assisted extraction method can significantly improve the extraction efficiency of wax gourd polysaccharide;the wax gourd polysaccharide had good antioxidant activity.