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目的筛选出适宜的血清及秋水仙碱的作用浓度及时间,使乳腺癌MCF-7细胞分别达到G0/G1和G2/M期同步化。方法采用血清饥饿法诱导MCF-7细胞G0/G1同步化,秋水仙碱诱导细胞G2/M期同步化,流式细胞仪检测细胞各期分布情况。结果处理24 h后,1%血清浓度组G0/G1期细胞分别达到(72. 96±0.84)%,1. 0μg/mL秋水仙碱处理组G2/M期细胞分别占总细胞数(73. 67±1. 77)%。处理36 h后,1%血清浓度及秋水仙碱实验组细胞发生凋亡。结论MCF-7在血清浓度为1%处理24 h后完成G0/G1同步化,在1. 0μg/mL的秋水仙碱处理24 h后完成G2/M期同步化。
Abstract:Objective This experiment is conducted to select the appropriate concentration and time of serum and colchicine to synchronize breast cancer MCF-7 cells at G0/G1 and G2/M phases,respectively. Methods MCF-7 was treated with different serum concentration to induce cell at G0/G1 stage synchronization,and treated with colchicine reagents to induce cell at G2/M synchronization.Cell cycle distribution was analyzed by flow cytometry for 12,24,36 h. Results After 24 hours treated,in 1% serum concentration group,the percentage of MCF-7 at G0/G1 accounted for(72. 96 ±0. 84)%;in 1. 0 μg/mL colchicine reagent group,the percentage of MCF-7 at G2/M stage was(73. 67 ±1. 77)%. 36 hours after treatment,in experiment groups of 1% serum and the group of colchicine,cell apoptosis was induced. Conclusion In experimental groups,1% serum treating and 1. 0 μg/mL colchicine regents treating for 24 h can basically completed cell cycle synchronization at G0/G1 and G2/M phases,respectively.
[1] HUANG H T,FAVA A,GUHR T,et al. A methodology for exploring biomarker-phenotype associations:application to flow cytometry data and systemic sclerosis clinical manifestation[J]. BMC Bioinformatics,2015,16:293.
[2] SANTEGOETS S J A M,DIJKGRAAF E M,BATTAGLIA A,et al. Monitoring regulatory T cell in clinical samples:consensus on an essential marker set and gating strategy for regulatory T cell analysis by flow cytometry[J]. Cancer Immunol Immunother,2015,64:1271-1286.
[3] BENEDEK G,MEZA-ROMERO R,BOURDETTE D,et al. The use of flow cytometry to assess a novel drug efficacy in multiple sclerosis[J]. Metab Brain Dis,2015,30:877-884.
[4]韩鸿鹏,宋纯鹏.流式细胞术在植物生物学研究中应用进展[J].北方园艺,2015(15):178-181.
[5] SHI M,SPURGEON S,PRESS R,et al. MYD88 mutation analysis of a rare composite chronic lymphocyte leukemia and lymphoplasmacytic lymohoma by cytometry cell sorting[J]. Ann Hematol,2015,94(11):1941-1944.
[6]方娟,阮绪芝,张相华,等. FAM92A1-289在不同增值能力组织及不同周期时相中的表达和意义[J].广东医学,2015,36(14):2187-2190.
[7] LU Y,CHEN J,XIAO M,et al. An overview of tubulin inhibitors that interact with the colchicine binding site[J]. Pharm Res,2012,29(11):2943-2971.
基本信息:
DOI:10.16389/j.cnki.cn42-1737/n.2019.05.011
中图分类号:R737.9
引用信息:
[1]周晓叶,袁俊勇,王安,等.应用流式细胞术研究乳腺癌MCF-7细胞周期同步化[J].江汉大学学报(自然科学版),2019,47(05):454-458.DOI:10.16389/j.cnki.cn42-1737/n.2019.05.011.
基金信息:
湖北省教育厅科学技术研究项目(B2015235); 武汉市卫计委医疗卫生科研项目(WXB14B26)