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麦冬是一种著名的传统药用植物。它在提高免疫功能方面发挥着重要的作用，但是对其作用机制的了解还不是很全面。首先以自由基清除率为指标，分析麦冬多糖的体外抗氧化活性，然后再通过H2O2诱导NIH/3T3小鼠胚胎成纤维细胞损伤的模型，进一步研究麦冬多糖在细胞层面的抗氧化效果。同时，通过考查麦冬多糖抑制葡萄糖苷酶活性来初步阐明其在细胞免疫功能方面的作用。实验结果表明，麦冬多糖在体外具有很好的抗氧化效果，当麦冬多糖的浓度大于100μg/mL时，对DPPH自由基的清除效果与阳性对照(抗坏血酸)相当。同时当麦冬多糖的浓度大于20μg/mL时，其羟基自由基的清除率甚至高于阳性对照。在体内H2O2诱导的NIH/3T3细胞损伤模型中，麦冬多糖的预处理可以很好地降低NIH/3T3细胞的死亡率，降低单线态氧的产生，以及炎症因子的表达量，进一步证明了麦冬多糖在细胞体内的抗氧化效果。最后在葡萄糖苷酶活性实验中，麦冬多糖对葡萄糖苷酶的抑制活性随着浓度的增加而增强，在浓度为200μg/mL时，抑制活性与阳性对照(阿卡波糖)基本持平。实验结果说明了麦冬多糖可作为潜在的抗氧化剂和降血糖功能性食品。
Abstract：Ophiopogon japonicus is a famous traditional medicinal plant,which plays a significant role in improving cellular immune functions.However,the understanding of its action mechanism is not very comprehensive.Firstly,a free radical scavenging assay was applied to analyze its antioxidant capacity in vitro,then H2O2 was used to establish the NIH/3T3 mouse embryonic fibroblasts cell injury model and to further study the intracellular antioxidant effect of Ophiopogon japonicus polysaccharides.Meanwhile,to preliminarily clarify the role of Ophiopogon japonicus polysaccharides in cellular immune function,the activity of α-glucosidase was investigated.The results demonstrated that when the concentration of Ophiopogon japonicus polysaccharides was above 100μg/mL,its DPPH radical activity was the same as the positive control of ascorbic acid,while the hydroxyl radical scavenging capacity was slightly higher than that of ascorbic acid only when the concentration higher than 20μg/mL.All these data illustrated the strong antioxidant activity of Ophiopogon japonicus polysaccharides in vitro.In the NIH/3T3 cell injury model induced by H2O2,Ophiopogon japonicus polysaccharides could well reduce the mortality of NIH/3T3 cells,the production of singlet oxygen,and the expression of inflammatory factors,which further proved the antioxidant effect of Ophiopogon japonicus polysaccharides.In theα-glucosidase activity assay,the inhibition rate of Ophiopogon japonicus polysaccharides was in a concentration-dependent manner,its inhibition capacity was equivalent to that of acarbose at a concentration higher than 200μg/mL.These results indicated that Ophiopogon japonicus polysaccharides could be applied as a potential antioxidant therapeutics and hypoglycemic functional food.
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基本信息:
DOI：10.16389/j.cnki.cn42-1737/n.2023.03.006
中图分类号:R285
引用信息:
[1]文丹,付成,丁菲.麦冬多糖的抗氧化、降血糖活性研究[J].江汉大学学报(自然科学版),2023,51(03):55-67.DOI:10.16389/j.cnki.cn42-1737/n.2023.03.006.
基金信息:
国家自然科学基金资助项目（20160081）