江汉大学学报（自然科学版）

本试验以MS作为基本培养基,以‘惠农5号’甜叶菊试管苗茎段为实验材料,研究了6-BA、KT、Ad 3种外源激素的6个浓度的溶液与0. 1 mg/L NAA处理组合对试管苗增殖及生根的影响。试验结果表明,添加适宜浓度的外源激素作用效果是很明显的,其中适用于甜叶菊试管苗的继代培养形成的培养基为MS+6-BA 1. 0 mg/L+NAA 0. 1 mg/L+糖30 g/L+琼脂粉5. 5 g/L;适合甜叶菊试管苗增殖的培养基为MS+6-BA 2. 0 mg/L+NAA 0. 1 mg/L+糖30 g/L+琼脂粉5. 5 g/L,增殖系数可达3. 50;试管苗最佳生根培养基为1/2 MS+NAA 0. 1 mg/L+糖20 g/L+琼脂粉5. 5 g/L。不同外源激素种类、不同浓度及不同的处理组合对甜叶菊试管苗增殖及生根的影响不同。

In this experiment,MS was used as the basic medium and the stem segments of′huinong No. 5′ stevia test-tube seedlings were used as experimental materials. The effects of the treatment combination of 6-BA,KT,Ad three exogenous hormone solutions at 6 concentrations and 0. 1 mg/L NAA on the proliferation and rooting of test-tube seedlings were studied. The results showed that the effects of adding an appropriate concentration of exogenous hormone were obvious. The medium suitable for the secondary culture of stevia test-tube seedlings was MS + 6-BA 1. 0 mg/L + NAA 0. 1 mg/L + sugar 30 g/L +AGAR powder 5. 5 g/L. The medium suitable for the proliferation of stevia test-tube seedlings was MS + 6-BA 2. 0 mg/L + NAA 0. 1 mg/L + sugar 30 g/L + AGAR powder 5. 5 g/L,the proliferation coefficient can reach 3. 50. The optimal rooting medium for test-tube seedlings was 1/2 MS + NAA 0. 1 mg/L + sugar 20 g/L + AGAR powder5. 5 g/L. Different kinds of exogenous hormones,different concentrations and different combinations of treatments have different effects on the proliferation and rooting of stevia test-tube seedlings.