江汉大学学报（自然科学版）

目的 观察中药复方制剂敏脱乐配合火针疗法治疗慢性湿疹的临床疗效，并进一步探讨可能的作用机制。方法 选取临床病例286例，随机双盲原则分为对照组和观察组。对照组给予西药常规抗过敏治疗，观察组给予中药方剂敏脱乐口服配合火针外治法。治疗4周之后评估疗效。进一步借助网络药理学和生物信息学理论，对两组患者治疗前后进行差异基因分析，选取INF-γ、IL-4及EOS、IgE作为疗效观察指标，探讨药物作用机制。结果 临床观察中观察组有效率89.7%，对照组有效率77.2%，观察组治疗效果优于对照组（P <0.05）。评估湿疹面积和严重程度指数（EASI）和皮肤病生活质量指数（DLQI）评分变化，观察组评分下降较对照组明显（P <0.05）。实验研究中，观察组较对照组治疗后IL-4、EOS、IgE降低明显，INF-γ升高明显（P <0.05）。结论 中药方剂敏脱乐配合火针疗法治疗慢性湿疹疗效确切，且能明显调节机体免疫机制，在湿疹治疗中起到积极作用，值得在临床中推广运用。

Objective To observe the clinical efficacy of the traditional Chinese medicine compound Mintuole combined with fire acupuncture in the treatment of chronic eczema,and further explore the possible action mechanism. Methods A total of 286 clinical cases were selected and randomly divided into the control group and the observation group according to the double-blind principle. The control group was given conventional anti-allergic treatment, and the observation group was given Mintuole orally combined with fireneedling external therapy. Efficacy was assessed after 4 weeks of treatment. Further,based on network pharmacology and bioinformatics' theory,the differential genes of the two groups of patients were analyzed before and after treatment. INF-γ,IL-4,EOS,and IgE were selected as the efficacy observation indexes to explore the mechanism of drug action. Results In the clinical observation,the effective rate of the observation group was 89. 7% and the effective rate of the control group was 77. 2%. The therapeutic effect of the observation group was better than that of the control group(P < 0. 05). Eczema area and severity index(EASI) and dermatology life quality index(DLQI) scores were evaluated. The scores in the observation group decreased significantly compared with those in the control group(P <0. 05). In the experimental study,compared with the control group,IL-4,EOS,and IgE in the observation group were significantly decreased, and INF-γ was significantly increased after treatment(P < 0. 05). Conclusion Mintuole combined with fire acupuncture therapy is effective in treating chronic eczema and can significantly regulate the body's immune mechanism.