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为研究外源表油菜素内酯(EBR)处理对牡丹耐热性的影响,以3年生‘凤丹’牡丹容器苗为材料,通过叶面喷施0. 1、0. 5、1. 0和1. 5 mg/L的EBR,并采用人工气候模拟热胁迫(40℃/35℃,昼/夜),测定‘凤丹’叶片的相关生理生化指标及超微结构。结果表明:外源EBR提高了‘凤丹’叶片的净光合速率,降低了‘凤丹’叶片的相对电导率,有效抑制丙二醛的积累,降低可溶性糖含量,提高可溶性蛋白含量,增强了超氧化物歧化酶(SOD)活性,减缓高温对‘凤丹’细胞的伤害,并使细胞保持较完整的结构,抑制了细胞膜的解体、叶绿体和线粒体的变形,适宜浓度的外源EBR处理可增强牡丹的耐热能力,以1. 0 mg/L浓度处理效果最佳。
Abstract：In order to study the effects of exogenous 2,4-epibrassinolide on the heat tolerance ofpeony,the 3-year-old Paeonia ostii ′ Fengdan′ container seedlings was taken as materials whichtreated with 0. 1,0. 5,1. 0,1. 5 mg/L of epibrassinolide through spraying them on the leaves,underthe artificial climate simulating the heat stress(40 ℃/35 ℃,day/night),the related physiologicaland biochemical indexes and ultrastructure in leaves of Paeonia ostii ′Fengdan′ were determined inthe experiment. The experimental results showed that: exogenous epibrassinolide increased netphotosynthetic rate and reduced the relative electrical conductivity of leaves of Paeonia ostii ′Fengdan′.The accumulation of malondialdehyde was inhibited effectively and the soluble sugar content wasdecreased. The content of soluble protein was increased and the superoxide dismutase(SOD)activitywas enhanced. The exogenous epibrassinolide could slow down the rate of Paeonia ostii ′Fengdan′ celldamage by the high temperature and let the cells remain relatively intact structure. It could also inhibitthe disintegration of cell membrane,the deformation of chloroplast and mitochondrial. Appropriateconcentration of exogenous epibrassinolide can enhance the heat resistance of peony,and theconcentration of 1. 0 mg/L is the best.
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基本信息:
DOI：10.16389/j.cnki.cn42-1737/n.2018.05.009
中图分类号:S685.11
引用信息:
[1]任增斌,陈法志,舒常庆,等.外源表油菜素内酯对牡丹耐热性的影响[J].江汉大学学报(自然科学版),2018,46(05):446-453.DOI:10.16389/j.cnki.cn42-1737/n.2018.05.009.
基金信息:
湖北省技术创新专项重大项目(2018ABA073)
2018-10-18
2018-10-18
2018-10-18